久久久久亚洲AV无码专区体验,亚洲一区二区三区国产精华液,欧美与黑人午夜性猛交久久久,少妇高潮喷水久久久久久久久

免費(fèi)咨詢熱線:
15522676233
技術(shù)文章
首頁(yè) > 技術(shù)中心 > PEI25K轉(zhuǎn)染試劑的應(yīng)用

PEI25K轉(zhuǎn)染試劑的應(yīng)用

 更新時(shí)間:2022-11-24 點(diǎn)擊量:979

PEI又稱(chēng)為線性化聚乙烯亞胺,是人工合成的含有高密度正電荷的有機(jī)大分子陽(yáng)離子聚合物。PEIDNA通過(guò)正負(fù)電荷的相互吸引形成復(fù)合物,這些復(fù)合物很容易通過(guò)內(nèi)吞作用進(jìn)入細(xì)胞。PEI-DNA復(fù)合物能很好地抵御溶酶體的降解作用,有效提高轉(zhuǎn)染效率。PEI可作用于大至135000個(gè)核苷酸的質(zhì)粒,所以較大的質(zhì)粒也可以成功轉(zhuǎn)染。目前市面上PEI轉(zhuǎn)染試劑有很多種,PolysciencesPEI線性轉(zhuǎn)染試劑適用于多種真核細(xì)胞,用途廣泛。

一、Polysciences線性PEI轉(zhuǎn)染試劑(PEI 25K)特性:

貨號(hào):23966-1

分子式:(CH2CH2NH)n

分子量:25000

外觀:白色至黃色粉末

熔點(diǎn):73-75

溶解性:溶于:熱水、低pH值的冷水、甲醇和乙醇;

不溶于:苯、YI醚和丙酮。

微信截圖_20221123145305.png

二、Polysciences聚乙烯亞胺轉(zhuǎn)染試劑(PEI 25K)的應(yīng)用:

遞送物質(zhì)
轉(zhuǎn)染細(xì)胞類(lèi)型轉(zhuǎn)染細(xì)胞舉例(包括但不限于)
DNA貼壁/懸浮細(xì)胞HEK293
CHO
HELA
SF9昆蟲(chóng)細(xì)胞
SF21昆蟲(chóng)細(xì)胞等真核細(xì)胞系
三、應(yīng)用Polysciences PEI線性轉(zhuǎn)染試劑發(fā)表的高分文章:

(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9.  IF19.160

試劑:PEI 25K應(yīng)用:DNA轉(zhuǎn)染人胚腎293S細(xì)胞。

(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54.  IF10.633

試劑:PEI 25K;應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染小鼠骨髓間充質(zhì)干細(xì)胞(D1)。

(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020.  IF6.630

試劑:PEI 25K;應(yīng)用:包裝質(zhì)粒pCMVdelta8.9、包膜質(zhì)粒VSV-GPD-1-GFP、PD-L1-GFP或空GFP sFFV轉(zhuǎn)移質(zhì)粒轉(zhuǎn)染HEK293T細(xì)胞

(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensormodified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276.  IF4.395

試劑:PEI 25K;應(yīng)用:轉(zhuǎn)染HEK293T細(xì)胞。

(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739.  IF:4.345

試劑:PEI 25K(23966-1);應(yīng)用:質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞

(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373.  IF3.602

試劑:PEI 25K(23966-1);應(yīng)用:shRNA-PLKO.1質(zhì)粒轉(zhuǎn)染HEK293FT細(xì)胞

(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132.  IF3.595

試劑:PEI 25K;應(yīng)用:質(zhì)粒DNA轉(zhuǎn)染懸浮生長(zhǎng)的CHO DG44細(xì)胞。

(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.

試劑:PEI 25K;應(yīng)用:DNA轉(zhuǎn)染HEK293T細(xì)胞。

(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.  

試劑:PEI 25K;應(yīng)用:將編碼fLuc基因的DNA遞送至小鼠。

(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.  

試劑:PEI MAX(24765)應(yīng)用:用質(zhì)粒DNA轉(zhuǎn)染ET801載體—幼倉(cāng)鼠腎來(lái)源細(xì)胞。

北京百奧創(chuàng)新科技有限公司提供多款Polysciences轉(zhuǎn)染試劑,具體信息請(qǐng)咨詢百奧創(chuàng)新客服。(Polysciences PEI 25K 23966-1現(xiàn)貨打折,*!機(jī)會(huì)有限,不要錯(cuò)過(guò)!)

微信截圖_20221123150211.png



中文字幕乱偷无码AV先锋蜜桃| 国产精品女同一区二区| 精品无码久久久久久久久水蜜桃| 最好朋友的老公上我了怎么办| fc2成年免费共享视频| WWW国产成人免费观看视频| 成人H视频在线观看| 一本色道dvd中文字幕蜜桃视频| 毛片亚洲AV无码精品国产午夜| 男男sm调教腐文| XXXXX性BBBBB欧美| 警察受被粗汉h玩松了尿进去双性| 精品久久久久久久久久中文字幕| 色戒假戏真做7分27秒视频| 久久精品国产亚洲AV麻豆蜜芽 | 人妻女友娇妻沉沦系列| 国产亚洲精品无码成人| 全是肉的高h短篇bl| 将军与娇妻各种做高h| 欧美变态口味重另类在线视频| 久久日本片精品AAAAA国产| 免费真人视频网站| 精品无码国产污污污免费网站| 性兴奋流出来白色粘稠液体| 国产亚洲精品精华液| 老师好大好爽我要喷水了视频| 教官趁她睡着吸允她的花蜜| 精品一区二区三区自拍图片区| 欧美最猛黑人XXXXX猛交| 无码成人性爽xo视频在线观看| 久久午夜夜伦鲁鲁片无码免费| 亚洲色偷偷色噜噜狠狠99网| 在线视频国产欧美另类| 国内精品久久久久久无码不卡 | 久久久久久A亚洲欧洲AV | 人与禽性视频77777| 久久精品国产亚洲AV成人| 欧美精品一区二区| 久久久久久久精品免费久精品蜜桃| 后入内射无码人妻一区| 久久久久亚洲精品无码蜜桃|